Double nanohole optical trapping: dynamics and protein-antibody co-trapping

Lab Chip. 2013 Jul 7;13(13):2563-8. doi: 10.1039/c3lc00003f. Epub 2013 Feb 22.

Abstract

A double nanohole in a metal film can optically trap nanoparticles such as polystyrene/silica spheres, encapsulated quantum dots and up-converting nanoparticles. Here we study the dynamics of trapped particles, showing a skewed distribution and low roll-off frequency that are indicative of Kramers-hopping at the nanoscale. Numerical simulations of trapped particles show a double-well potential normally found in Kramers-hopping systems, as well as providing quantitative agreement with the overall trapping potential. In addition, we demonstrate co-trapping of bovine serum albumin (BSA) with anti-BSA by sequential delivery in a microfluidic channel. This co-trapping opens up exciting possibilities for the study of protein interactions at the single particle level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / chemistry*
  • Antibodies / immunology
  • Microfluidic Analytical Techniques / instrumentation
  • Microfluidic Analytical Techniques / methods*
  • Nanoparticles / chemistry*
  • Optical Tweezers
  • Polystyrenes / chemistry
  • Protein Interaction Maps
  • Serum Albumin, Bovine / chemistry*
  • Serum Albumin, Bovine / immunology
  • Silicon Dioxide / chemistry

Substances

  • Antibodies
  • Polystyrenes
  • Serum Albumin, Bovine
  • Silicon Dioxide