Rapid and accurate identification of poliovirus strains used for vaccine production

Olaf E M Nijst; Justin J Mouthaan; Dirk R Mekkes; Edin Jusic; Harrie G A M van der Avoort; Bernard Metz

doi:10.1016/j.jviromet.2013.01.026

Rapid and accurate identification of poliovirus strains used for vaccine production

J Virol Methods. 2013 Apr;189(1):189-95. doi: 10.1016/j.jviromet.2013.01.026. Epub 2013 Feb 19.

Authors

Olaf E M Nijst¹, Justin J Mouthaan, Dirk R Mekkes, Edin Jusic, Harrie G A M van der Avoort, Bernard Metz

Affiliation

¹ National Institute for Public Health and the Environment, Antonie van Leeuwenhoeklaan 9, 3720 BA Bilthoven, The Netherlands.

PMID: 23434540
DOI: 10.1016/j.jviromet.2013.01.026

Abstract

In the context of eradication of poliomyelitis the World Health Organization stimulates the development of inactivated polio vaccines based on attenuated virus strains. In addition to vaccine development, tests have to be designed to assess the vaccine quality. An important test is the identification test for poliovirus strains that are used for the vaccine production. A rapid and accurate PCR method with fluorescent probes has been developed to identify unequivocally the vaccine-specific poliovirus strains, such as Mahoney, MEF-1, Saukett H, Sabin type 1, Sabin type 2 and Sabin type 3.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Viral / immunology
Cell Line
Humans
Poliomyelitis / immunology
Poliomyelitis / prevention & control*
Poliomyelitis / virology
Poliovirus / classification*
Poliovirus / genetics*
Poliovirus / immunology
Poliovirus Vaccine, Inactivated / immunology*
Poliovirus Vaccine, Oral / immunology*
Polymerase Chain Reaction
RNA, Viral / analysis
RNA, Viral / genetics

Substances

Antibodies, Viral
Poliovirus Vaccine, Inactivated
Poliovirus Vaccine, Oral
RNA, Viral