Abstract
To investigate echinococcosis in co-endemic regions, three polymerase chain reaction (PCR) assays based on the amplification of a fragment within the NADH dehydrogenase subunit 1 (ND1) mitochondrial gene were optimized for the detection of Echinococcus shiquicus, Echinococcus granulosus G1, and Echinococcus multilocularis DNA derived from parasite tissue or canid fecal samples. Specificity using parasite tissue-derived DNA was found to be 100% except for E. shiquicus primers that faintly detected E. equinus DNA. Sensitivity of the three assays for DNA detection was between 2 and 10 pg. Ethanol precipitation of negative PCR fecal samples was used to eliminate false negatives and served to increase sensitivity as exemplified by an increase in detection from 0% to 89% of E. shiquicus coproDNA using necropsy-positive fox samples.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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China / epidemiology
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DNA, Helminth / analysis*
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DNA, Helminth / genetics
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DNA, Mitochondrial / analysis
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DNA, Mitochondrial / genetics
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Dogs / parasitology
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Echinococcosis / epidemiology
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Echinococcosis / parasitology
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Echinococcosis / veterinary
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Echinococcus granulosus / classification*
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Echinococcus granulosus / enzymology
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Echinococcus granulosus / genetics
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Echinococcus multilocularis / classification*
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Echinococcus multilocularis / enzymology
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Echinococcus multilocularis / genetics
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Endemic Diseases*
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Ethanol / chemistry
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Feces / parasitology
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Foxes / parasitology
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Genes, Mitochondrial
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Genotype
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Genotyping Techniques / methods
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Mitochondria / genetics
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NADH Dehydrogenase / genetics
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Sequence Analysis, DNA
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Tibet / epidemiology
Substances
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DNA, Helminth
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DNA, Mitochondrial
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Ethanol
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NADH Dehydrogenase