Kinetic analysis of bile salt passage across a dialysis membrane in the presence of cereal soluble dietary fibre polymers

Food Chem. 2012 Oct 15;134(4):2007-13. doi: 10.1016/j.foodchem.2012.03.131. Epub 2012 Apr 9.

Abstract

The kinetics of passage of a model bile salt and complete porcine bile across a dialysis membrane, in the presence and absence of two cereal-derived soluble dietary fibre polysaccharides, were studied as a model for passage across the unstirred water layer that lines the small intestine. A first-order kinetic analysis allowed rate coefficients to be derived which quantified the effectiveness of barley mixed linkage β-glucan and wheat arabinoxylan in retarding the transport of bile. For both, a model bile salt and complete porcine bile, rate coefficients decreased with both concentration and viscosity. A combination of viscosity and molecular interaction effects is suggested to control the effect of the two polysaccharides on the transport of bile.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bile Acids and Salts / chemistry
  • Bile Acids and Salts / metabolism*
  • Biopolymers / chemistry
  • Biopolymers / metabolism
  • Dialysis
  • Dietary Fiber / analysis
  • Dietary Fiber / metabolism*
  • Hordeum / metabolism*
  • Intestinal Mucosa / metabolism*
  • Kinetics
  • Models, Biological
  • Permeability
  • Swine
  • Triticum / metabolism*
  • Viscosity
  • Xylans / chemistry
  • Xylans / metabolism
  • beta-Glucans / chemistry
  • beta-Glucans / metabolism

Substances

  • Bile Acids and Salts
  • Biopolymers
  • Dietary Fiber
  • Xylans
  • beta-Glucans
  • arabinoxylan