Abstract
Several intestinal damage models have been developed using zebrafish, with the aim of recapitulating aspects of human inflammatory bowel disease (IBD). These experimentally induced inflammation models have utilized immersion exposure to an array of colitogenic agents (including live bacteria, bacterial products, and chemicals) to induce varying severity of inflammation. This technical report describes methods used to generate two chemically induced intestinal damage models using either dextran sodium sulfate (DSS) or trinitrobenzene sulfonic acid (TNBS). Methods to monitor intestinal damage and inflammatory processes, and chemical-genetic methods to manipulate the host response to injury are also described.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Alcian Blue / metabolism
-
Animals
-
Anti-Bacterial Agents / administration & dosage
-
Anti-Inflammatory Agents / administration & dosage
-
Dextran Sulfate / pharmacology*
-
Disease Models, Animal*
-
Drug Discovery
-
Enterocolitis / chemically induced*
-
Enterocolitis / drug therapy
-
Enterocolitis / immunology
-
Enterocolitis / microbiology
-
Flow Cytometry
-
Humans
-
Immersion
-
Inflammation / chemically induced*
-
Inflammation / drug therapy
-
Inflammation / immunology
-
Inflammation / microbiology
-
Inflammatory Bowel Diseases / chemically induced*
-
Inflammatory Bowel Diseases / drug therapy
-
Inflammatory Bowel Diseases / immunology
-
Inflammatory Bowel Diseases / microbiology
-
Interleukin-23 / genetics
-
Interleukin-23 / immunology
-
Interleukin-23 / metabolism
-
Intestines / microbiology
-
Intestines / pathology
-
Larva
-
Microinjections
-
Morpholinos / administration & dosage
-
Neutral Red / metabolism
-
Neutrophils / immunology
-
Nitric Oxide / metabolism
-
Optical Imaging
-
Polymerase Chain Reaction
-
Trinitrobenzenesulfonic Acid / pharmacology*
-
Zebrafish Proteins / genetics
-
Zebrafish Proteins / immunology
-
Zebrafish Proteins / metabolism
-
Zebrafish*
Substances
-
Anti-Bacterial Agents
-
Anti-Inflammatory Agents
-
Interleukin-23
-
Morpholinos
-
Zebrafish Proteins
-
Neutral Red
-
Nitric Oxide
-
Trinitrobenzenesulfonic Acid
-
Dextran Sulfate
-
Alcian Blue