Abstract
We retrospectively confirmed 2 cases of human Anaplasma phagocytophilum infection. Patient blood samples contained unique p44/msp2 for the pathogen, and antibodies bound to A. phagocytophilum antigens propagated in THP-1 rather than HL60 cells. Unless both cell lines are used for serodiagnosis of rickettsiosis-like infections, cases of human granulocytic anaplasmosis could go undetected.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Anaplasma phagocytophilum / genetics*
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Anaplasmosis / blood
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Anaplasmosis / diagnosis*
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Anaplasmosis / drug therapy
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Anaplasmosis / immunology
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Antibodies, Bacterial / blood
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Bacterial Outer Membrane Proteins / immunology
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DNA, Bacterial / blood
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification
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Ehrlichiosis / blood
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Ehrlichiosis / diagnosis*
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Ehrlichiosis / drug therapy
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Ehrlichiosis / immunology
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Genes, Bacterial
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HL-60 Cells
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Humans
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Japan
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Male
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Middle Aged
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Molecular Diagnostic Techniques
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Molecular Typing
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Phylogeny
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RNA, Ribosomal, 16S / genetics
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Retrospective Studies
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Serologic Tests
Substances
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Antibodies, Bacterial
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Bacterial Outer Membrane Proteins
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DNA, Bacterial
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RNA, Ribosomal, 16S