Dimerization of complement factor H-related proteins modulates complement activation in vivo

Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4685-90. doi: 10.1073/pnas.1219260110. Epub 2013 Mar 4.

Abstract

The complement system is a key component regulation influences susceptibility to age-related macular degeneration, meningitis, and kidney disease. Variation includes genomic rearrangements within the complement factor H-related (CFHR) locus. Elucidating the mechanism underlying these associations has been hindered by the lack of understanding of the biological role of CFHR proteins. Here we present unique structural data demonstrating that three of the CFHR proteins contain a shared dimerization motif and that this hitherto unrecognized structural property enables formation of both homodimers and heterodimers. Dimerization confers avidity for tissue-bound complement fragments and enables these proteins to efficiently compete with the physiological complement inhibitor, complement factor H (CFH), for ligand binding. Our data demonstrate that these CFHR proteins function as competitive antagonists of CFH to modulate complement activation in vivo and explain why variation in the CFHRs predisposes to disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Complement Activation / physiology*
  • Complement System Proteins* / chemistry
  • Complement System Proteins* / genetics
  • Complement System Proteins* / metabolism
  • Dimerization*
  • Genetic Loci*
  • Humans
  • Protein Structure, Quaternary
  • Protein Structure, Tertiary
  • Structure Collapse

Substances

  • Complement System Proteins

Associated data

  • PDB/3ZD1
  • PDB/3ZD2