Abstract
1-(5-Isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride (H-7), a potent and selective inhibitor of protein kinase C (PKC), inhibited PHA-stimulated bovine peripheral blood mononuclear cell (PBMC) proliferation, interleukin-2 (IL-2) production, and cytosolic PKC activity without affecting the cell viabilities. Presence of exogenous cytokines, such as purified human IL-2 or recombinant bovine IL-2 (rbovIL-2), reversed the H-7 inhibitory effects on PHA-stimulated PBMC proliferation. We conclude that the PKC enzyme plays an important role as a second messenger in bovine PBMC proliferation in the early stages of cell activation.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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Analysis of Variance
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Animals
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Cattle
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Cell Division / drug effects
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Cell Survival / drug effects
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Cells, Cultured
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Cytosol / enzymology
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Interleukin-2 / biosynthesis*
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Interleukin-2 / pharmacology
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Isoquinolines / antagonists & inhibitors
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Isoquinolines / pharmacology*
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Leukocytes, Mononuclear / drug effects*
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Leukocytes, Mononuclear / immunology
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Lymphocyte Activation
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Phytohemagglutinins / immunology
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Piperazines / antagonists & inhibitors
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Piperazines / pharmacology*
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Protein Kinase C / antagonists & inhibitors*
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Recombinant Proteins
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Sulfonamides*
Substances
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Interleukin-2
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Isoquinolines
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Phytohemagglutinins
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Piperazines
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Recombinant Proteins
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Sulfonamides
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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N-(2-guanidinoethyl)-5-isoquinolinesulfonamide
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Protein Kinase C