Strategies for the discovery and engineering of enzymes for biocatalysis

Timo Davids; Marlen Schmidt; Dominique Böttcher; Uwe T Bornscheuer

doi:10.1016/j.cbpa.2013.02.022

Strategies for the discovery and engineering of enzymes for biocatalysis

Curr Opin Chem Biol. 2013 Apr;17(2):215-20. doi: 10.1016/j.cbpa.2013.02.022. Epub 2013 Mar 21.

Authors

Timo Davids¹, Marlen Schmidt, Dominique Böttcher, Uwe T Bornscheuer

Affiliation

¹ Institute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, University of Greifswald, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany.

PMID: 23523243
DOI: 10.1016/j.cbpa.2013.02.022

Abstract

Protein engineering is the most important method to overcome the limitations of natural enzymes as biocatalysts. The past few years have seen a tremendous increase in novel concepts to facilitate the design of mutant libraries for focused directed evolution mostly guided by advanced bioinformatic tools. In addition, advanced high-throughput methods were developed using, for example, FACS analysis or microfluidic systems. These achievements significantly facilitate the tailor-made design of enzymes to make them suitable for industrial applications.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biocatalysis
Computational Biology
Databases, Protein
Directed Molecular Evolution
Enzymes / chemistry*
Enzymes / genetics
Enzymes / metabolism*
High-Throughput Screening Assays
Protein Engineering / methods*
Software

Substances

Enzymes