Downregulation of miRNA-128 sensitises breast cancer cell to chemodrugs by targeting Bax

Shufeng Ji; Guoli Shao; Xiaobin Lv; Yuan Liu; Yingfang Fan; Aiguo Wu; Haiyan Hu

doi:10.1002/cbin.10100

Downregulation of miRNA-128 sensitises breast cancer cell to chemodrugs by targeting Bax

Cell Biol Int. 2013 Jul;37(7):653-8. doi: 10.1002/cbin.10100. Epub 2013 May 8.

Authors

Shufeng Ji¹, Guoli Shao, Xiaobin Lv, Yuan Liu, Yingfang Fan, Aiguo Wu, Haiyan Hu

Affiliation

¹ Department of General Surgery, Zhujiang Hospital of Nanfang Medical University, No 253 Industrial Avenue, Haizhu District of Guangzhou City, Guangdong Province 501282, China.

PMID: 23526655
DOI: 10.1002/cbin.10100

Abstract

miR-128 is more highly expressed in drug-resistant breast cancer samples than in drug-sensitive samples. We have confirmed that Bax is the target of miR-128 by negative post-transcriptional regulation. miR-128 and Bax were detected in the breast cancer cell line, MDA-MB-231, which was then transfected with miR-128 MIMIC (precursor of miR-128) or AMO (antisense-miR-128 oligonucleotides). After transfection, the chemosensitivity of MDA-MB-231 cell was up-regulated with increasing of Bax and inhibition of miR-128.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Apoptosis
Breast Neoplasms / metabolism
Breast Neoplasms / pathology
Cell Line, Tumor
Down-Regulation
Drug Resistance, Neoplasm
Female
Humans
MicroRNAs / antagonists & inhibitors
MicroRNAs / metabolism*
Middle Aged
Oligonucleotides, Antisense / metabolism
RNA Interference
RNA, Small Interfering / metabolism
bcl-2-Associated X Protein / antagonists & inhibitors
bcl-2-Associated X Protein / genetics
bcl-2-Associated X Protein / metabolism*

Substances

MIRN128 microRNA, human
MicroRNAs
Oligonucleotides, Antisense
RNA, Small Interfering
bcl-2-Associated X Protein