tert-Butyl hydroperoxide-induced injury in isolated rat hepatocytes: a model for studying anti-hepatotoxic crude drugs

Planta Med. 1990 Apr;56(2):171-4. doi: 10.1055/s-2006-960918.

Abstract

Tert-butyl hydroperoxide induces in freshly isolated rat hepatocytes malonaldehyde formation and lacticodehydrogenase and aspartate amino-transferase leakage. This model, when adapted to crude extracts, permits the demonstration of both anti-lipoperoxidant and antihepatotoxic activity of reference products like quercetin and silymarin and plant extracts like Rosmarinus officinalis and Eschscholzia californica.

MeSH terms

  • Animals
  • Aspartate Aminotransferases / metabolism
  • L-Lactate Dehydrogenase / metabolism
  • Liver / cytology
  • Liver / drug effects*
  • Liver / metabolism
  • Magnoliopsida / analysis
  • Male
  • Malondialdehyde / metabolism
  • Peroxides / toxicity*
  • Plant Extracts / pharmacology
  • Promethazine / pharmacology
  • Quercetin / pharmacology
  • Rats
  • Silymarin / pharmacology
  • tert-Butylhydroperoxide

Substances

  • Peroxides
  • Plant Extracts
  • Silymarin
  • Malondialdehyde
  • tert-Butylhydroperoxide
  • Quercetin
  • L-Lactate Dehydrogenase
  • Aspartate Aminotransferases
  • Promethazine