A novel metallo-β-lactamase, IMP-34, in Klebsiella isolates with decreased resistance to imipenem

Norifumi Shigemoto; Shizuo Kayama; Ryuichi Kuwahara; Junzo Hisatsune; Fuminori Kato; Hisaaki Nishio; Katsutoshi Yamasaki; Yasunao Wada; Taijiro Sueda; Hiroki Ohge; Motoyuki Sugai

doi:10.1016/j.diagmicrobio.2013.02.030

A novel metallo-β-lactamase, IMP-34, in Klebsiella isolates with decreased resistance to imipenem

Diagn Microbiol Infect Dis. 2013 May;76(1):119-21. doi: 10.1016/j.diagmicrobio.2013.02.030. Epub 2013 Mar 27.

Authors

Norifumi Shigemoto¹, Shizuo Kayama, Ryuichi Kuwahara, Junzo Hisatsune, Fuminori Kato, Hisaaki Nishio, Katsutoshi Yamasaki, Yasunao Wada, Taijiro Sueda, Hiroki Ohge, Motoyuki Sugai

Affiliation

¹ Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, Hiroshima 734-8551, Japan.

PMID: 23541689
DOI: 10.1016/j.diagmicrobio.2013.02.030

Abstract

We investigated 5 metallo-β-lactamase (MBL)-positive Klebsiella isolates from Japan showing intermediate resistance to imipenem. Sequencing of the MBL gene identified a novel variant of IMP-1 with a single amino acid substitution, Glu87Gly. This variant is designated as IMP-34 where blaIMP-34 is located on a transmissible plasmid.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Drug Resistance, Multiple, Bacterial / genetics*
Imipenem / pharmacology*
Japan
Klebsiella / drug effects
Klebsiella / enzymology*
Klebsiella / genetics
Klebsiella / isolation & purification
Microbial Sensitivity Tests
Plasmids
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
Imipenem
beta-Lactamases