Cilostazol protects vessels against hyperglycemic injury and accelerates healing after implantation of drug-eluting stent in a type 1 diabetes mellitus rat aorta stent model

Atherosclerosis. 2013 Jun;228(2):332-8. doi: 10.1016/j.atherosclerosis.2013.03.008. Epub 2013 Mar 20.

Abstract

Objective: Cilostazol, a selective phosphodiesterase-3 (PDE-3) inhibitor, can effectively suppress platelet activation and attenuate the increase in carotid intima-media thickness in diabetes mellitus (DM) patients. Therefore, we investigated whether cilostazol had effects on the healing process after implantation of a drug-eluting stent (DES) in a rat model of type 1 DM.

Methods and results: Streptozotocin-induced DM rats were divided into 2 groups in which cilostazol (30 mg/kg/day; DM-Cilostazol) or vehicle (DM-Vehicle) was orally administered. Age-matched rats treated with the vehicle were used as a control group (NDM-Vehicle). After 4 weeks, cilostazol changed the expression of vascular cell adhesion molecule and intercellular adhesion molecule and the apoptotic cell ratio of the media (DM-Vehicle: 53.5 ± 9.8%, DM-Cilostazol: 26.4 ± 8.3%, p < 0.05) in the aortic wall. Also, in a modified aortic ring test, cilostazol preserved the angiogenic potential of the aorta ([height of the sprouting tubes] DM-Vehicle: 0 ± 0 μm, DM-Cilostazol: 344.6 ± 236.8 μm, p < 0.05). After implantation of paclitaxel-eluting stents (PES) in rats treated with cilostazol or vehicle, thrombus formation, deposition of fibrin, and infiltration of inflammatory cells were attenuated by cilostazol. In particular, the re-endothelialization by von Willebrand factor expression in the DM-PES-Cilostazol group was enhanced compared with that in the DM-PES-Vehicle group.

Conclusion: Cilostazol has potential for protecting vessels against hyperglycemic injury and for accelerating the healing process after implantation of DES.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Angioplasty, Balloon / adverse effects
  • Angioplasty, Balloon / instrumentation*
  • Animals
  • Aorta / drug effects*
  • Aorta / metabolism
  • Aorta / pathology
  • Aortic Diseases / blood
  • Aortic Diseases / etiology
  • Aortic Diseases / pathology
  • Aortic Diseases / therapy*
  • Apoptosis / drug effects
  • Blood Glucose / metabolism*
  • Cilostazol
  • Diabetes Mellitus, Experimental / blood
  • Diabetes Mellitus, Experimental / complications
  • Diabetes Mellitus, Experimental / drug therapy*
  • Diabetes Mellitus, Type 1 / blood
  • Diabetes Mellitus, Type 1 / complications
  • Diabetes Mellitus, Type 1 / drug therapy*
  • Diabetic Angiopathies / blood
  • Diabetic Angiopathies / etiology
  • Diabetic Angiopathies / pathology
  • Diabetic Angiopathies / therapy*
  • Drug-Eluting Stents*
  • Endothelial Cells / drug effects
  • Endothelial Cells / metabolism
  • Endothelial Cells / pathology
  • Intercellular Adhesion Molecule-1 / metabolism
  • Male
  • Neovascularization, Pathologic
  • Paclitaxel / administration & dosage
  • Phosphodiesterase 3 Inhibitors / administration & dosage
  • Phosphodiesterase 3 Inhibitors / pharmacology*
  • Prosthesis Design
  • Rats
  • Rats, Sprague-Dawley
  • Tetrazoles / administration & dosage
  • Tetrazoles / pharmacology*
  • Thrombosis / blood
  • Thrombosis / etiology
  • Thrombosis / pathology
  • Thrombosis / prevention & control
  • Time Factors
  • Vascular Cell Adhesion Molecule-1 / metabolism
  • Wound Healing / drug effects*
  • von Willebrand Factor / metabolism

Substances

  • Blood Glucose
  • Phosphodiesterase 3 Inhibitors
  • Tetrazoles
  • Vascular Cell Adhesion Molecule-1
  • von Willebrand Factor
  • Intercellular Adhesion Molecule-1
  • Cilostazol
  • Paclitaxel