In vitro and computational studies of transdermal perfusion of nanoformulations containing a large molecular weight protein

Madalena Martins; Nuno G Azoia; Artur Ribeiro; Ulyana Shimanovich; Carla Silva; Artur Cavaco-Paulo

doi:10.1016/j.colsurfb.2013.02.032

In vitro and computational studies of transdermal perfusion of nanoformulations containing a large molecular weight protein

Colloids Surf B Biointerfaces. 2013 Aug 1:108:271-8. doi: 10.1016/j.colsurfb.2013.02.032. Epub 2013 Mar 4.

Authors

Madalena Martins¹, Nuno G Azoia, Artur Ribeiro, Ulyana Shimanovich, Carla Silva, Artur Cavaco-Paulo

Affiliation

¹ IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.

PMID: 23563294
DOI: 10.1016/j.colsurfb.2013.02.032

Abstract

Transdermal perfusion of a large protein is reported for the first time, using a nanoemulsion of bovine serum albumin (66kDa) of 160nm prepared by a solid-in-oil (S/O) process. Molecular dynamics simulations confirmed skin permeation by these formulations, with integration of the protein into the lipid bilayers. These results demonstrate the real possibility of delivering large proteins transdermally for a range of medical and cosmetic applications.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Administration, Cutaneous
Animals
Cattle
Emulsions
Fluorescent Dyes
Hydrophobic and Hydrophilic Interactions
Kinetics
Lipid Bilayers / chemistry*
Molecular Dynamics Simulation
Molecular Weight
Myristates / chemistry*
Nanostructures / chemistry*
Serum Albumin, Bovine / chemistry
Serum Albumin, Bovine / metabolism*
Skin / metabolism*
Surface-Active Agents / chemistry*
Swine

Substances

Emulsions
Fluorescent Dyes
Lipid Bilayers
Myristates
Surface-Active Agents
isopropyl myristate
Serum Albumin, Bovine