Rational design of a novel propeptide for improving active production of Streptomyces griseus trypsin in Pichia pastoris

Zhenmin Ling; Yi Liu; Shaolei Teng; Zhen Kang; Jingjing Zhang; Jian Chen; Guocheng Du

doi:10.1128/AEM.00376-13

Rational design of a novel propeptide for improving active production of Streptomyces griseus trypsin in Pichia pastoris

Appl Environ Microbiol. 2013 Jun;79(12):3851-5. doi: 10.1128/AEM.00376-13. Epub 2013 Apr 5.

Authors

Zhenmin Ling¹, Yi Liu, Shaolei Teng, Zhen Kang, Jingjing Zhang, Jian Chen, Guocheng Du

Affiliation

¹ Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.

Abstract

Applying in silico simulations and in vitro experiments, the amino acid proline was proved to have a profound influence on Streptomyces griseus trypsinogen, and the hydrogen bond between H(57) and D(102) was found to be crucial for trypsin activity. By introducing an artificial propeptide, IVEF, the titer of trypsin was increased 6.71-fold.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bioreactors*
Computer Simulation
Electrophoresis, Polyacrylamide Gel
Genetic Vectors / genetics
Hydrogen Bonding
Models, Molecular*
Pichia / metabolism*
Protein Precursors / administration & dosage
Protein Precursors / pharmacology*
Streptomyces griseus / chemistry
Streptomyces griseus / enzymology*
Trypsin / biosynthesis*
Trypsinogen / chemistry
Trypsinogen / metabolism*

Substances

Protein Precursors
Trypsinogen
Trypsin