An in vivo analysis of the effect and duration of treatment with botulinum toxin type A using digital image speckle correlation

Skin Res Technol. 2013 Aug;19(3):220-9. doi: 10.1111/srt.12010. Epub 2013 Apr 8.

Abstract

Background: Use of Botulinum toxin type A (BTX-A) for facial wrinkles is well-documented, but current methods of subjective evaluation by clinicians and patients fail to objectively quantify the magnitude and duration of facial muscle paralysis.

Objective: (a) Determine the locus of facial muscular tension; (b) Quantify and monitor muscular paralysis and subsequent return; (c) Continuously correlate the appearance of wrinkles and muscular tension using non-invasive digital image speckle correlation (DISC) to measure treatment efficacy; (d) Corroborate objective data with existing rating scales (subject global assessment and facial lines outcome-11).

Methods: Two sequential images of slight facial motion (frowning, raising eyebrows) are taken with a camera for n = 6 patients pre- and post-treatment at different time points up to 24 weeks. DISC processes the images to produce a vector map of muscular displacement to obtain spatially resolved information regarding facial tension.

Results: We observed maximum paralysis (≥70%) at 2 weeks, and the rate of recovery varied widely ranging from 2 to 5 months, with two patients continuing to exhibit reduced contraction at 24 weeks. Vector analysis of pre-treatment contraction correctly predicted injection site and illustrated lines of maximum tension.

Conclusions: Digital image speckle correlation can precisely track the degree of contraction of different muscle groups following BTX-A injection. It can help predict injection site, quantify muscle paralysis, and monitor the recovery following BTX-A injection. Results were found to be reproducible across six patients.

Keywords: botulinum toxin type A; digital image speckle correlation; facial paralysis; forehead; glabellar complex; wrinkles.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Botulinum Toxins, Type A / administration & dosage*
  • Dermoscopy / methods*
  • Facial Muscles / anatomy & histology*
  • Facial Muscles / drug effects*
  • Female
  • Humans
  • Image Interpretation, Computer-Assisted / methods
  • Injections, Intramuscular
  • Middle Aged
  • Neuromuscular Agents / administration & dosage
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Signal Processing, Computer-Assisted
  • Skin / anatomy & histology*
  • Skin / drug effects*
  • Skin Aging / drug effects*
  • Statistics as Topic
  • Subtraction Technique
  • Treatment Outcome

Substances

  • Neuromuscular Agents
  • Botulinum Toxins, Type A