A novel approach to automated genotyping of Mycobacterium tuberculosis using a panel of 15 MIRU VNTRs

Tomoshige Matsumoto; Yuriko Koshii; Kazu Sakane; Tomomi Murakawa; Yukio Hirayama; Hisako Yoshida; Masashi Kurokawa; Yoshitaka Tamura; Takayuki Nagai; Ichiro Kawase

doi:10.1016/j.mimet.2013.03.022

A novel approach to automated genotyping of Mycobacterium tuberculosis using a panel of 15 MIRU VNTRs

J Microbiol Methods. 2013 Jun;93(3):239-41. doi: 10.1016/j.mimet.2013.03.022. Epub 2013 Apr 6.

Authors

Tomoshige Matsumoto¹, Yuriko Koshii, Kazu Sakane, Tomomi Murakawa, Yukio Hirayama, Hisako Yoshida, Masashi Kurokawa, Yoshitaka Tamura, Takayuki Nagai, Ichiro Kawase

Affiliation

¹ Osaka Prefectural Medical Center for Respiratory and Allergic Diseases, Japan. [email protected]

PMID: 23566824
DOI: 10.1016/j.mimet.2013.03.022

Abstract

In this study, we present a new approach to variable number tandem repeats (VNTR) analysis using the QIAxcel capillary electrophoresis system and a software-integrated peak calling function. Allelic ladders representing 15 mycobacterial interspersed repetitive units (MIRU)-VNTR loci were used to define peak calling tables thereby enabling high precision Mycobacterium tuberculosis strain identification.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Automation, Laboratory / methods*
DNA, Bacterial / genetics*
Electrophoresis, Capillary / methods
Minisatellite Repeats*
Molecular Typing / methods*
Mycobacterium tuberculosis / classification
Mycobacterium tuberculosis / genetics*

Substances

DNA, Bacterial