Robust and highly-efficient differentiation of functional monocytic cells from human pluripotent stem cells under serum- and feeder cell-free conditions

Masakatsu D Yanagimachi; Akira Niwa; Takayuki Tanaka; Fumiko Honda-Ozaki; Seiko Nishimoto; Yuuki Murata; Takahiro Yasumi; Jun Ito; Shota Tomida; Koichi Oshima; Isao Asaka; Hiroaki Goto; Toshio Heike; Tatsutoshi Nakahata; Megumu K Saito

doi:10.1371/journal.pone.0059243

Robust and highly-efficient differentiation of functional monocytic cells from human pluripotent stem cells under serum- and feeder cell-free conditions

PLoS One. 2013;8(4):e59243. doi: 10.1371/journal.pone.0059243. Epub 2013 Apr 3.

Authors

Masakatsu D Yanagimachi¹, Akira Niwa, Takayuki Tanaka, Fumiko Honda-Ozaki, Seiko Nishimoto, Yuuki Murata, Takahiro Yasumi, Jun Ito, Shota Tomida, Koichi Oshima, Isao Asaka, Hiroaki Goto, Toshio Heike, Tatsutoshi Nakahata, Megumu K Saito

Affiliation

¹ Department of Clinilcal Application, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.

Abstract

Monocytic lineage cells (monocytes, macrophages and dendritic cells) play important roles in immune responses and are involved in various pathological conditions. The development of monocytic cells from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) is of particular interest because it provides an unlimited cell source for clinical application and basic research on disease pathology. Although the methods for monocytic cell differentiation from ESCs/iPSCs using embryonic body or feeder co-culture systems have already been established, these methods depend on the use of xenogeneic materials and, therefore, have a relatively poor-reproducibility. Here, we established a robust and highly-efficient method to differentiate functional monocytic cells from ESCs/iPSCs under serum- and feeder cell-free conditions. This method produced 1.3 × 10(6) ± 0.3 × 10(6) floating monocytes from approximately 30 clusters of ESCs/iPSCs 5-6 times per course of differentiation. Such monocytes could be differentiated into functional macrophages and dendritic cells. This method should be useful for regenerative medicine, disease-specific iPSC studies and drug discovery.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, Differentiation / metabolism
Cell Culture Techniques
Cell Differentiation*
Cells, Cultured
Culture Media, Serum-Free
Cytokines / metabolism
Dendritic Cells / cytology
Dendritic Cells / metabolism*
Embryonic Stem Cells / physiology*
Humans
Immunomagnetic Separation
Induced Pluripotent Stem Cells / physiology*
Macrophages / cytology
Macrophages / metabolism*
Phenotype

Substances

Antigens, Differentiation
Culture Media, Serum-Free
Cytokines

Grants and funding

Funding was provided by grants from the Ministry of Health, Labour and Welfare to TN, a grant from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) to TN, grants from the Leading Project of MEXT to TN, a grant from Funding Program for World-Leading Innovative Research and Development on Science and Technology (FIRST Program) of Japan Society for the Promotion of Science (JSPS) to TN, grants from JSPS to TN and MKS, grants from the Takeda foundation, Mitsubishi Pharma Research Foundation and Suzuken memorial foundation to MKS and grants from Grants-in-Aid for Scientific Research from Japan Society for the Promotion of Science from the Ministry of Education, Culture, Sports, Science, and Technology of Japan to MDY. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.