Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance

H Leung; U Lehtinen; R Karjalainen; D Skinner; P Tooley; S Leong; A Ellingboe

doi:10.1007/BF00334519

Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance

Curr Genet. 1990 May;17(5):409-11. doi: 10.1007/BF00334519.

Authors

H Leung¹, U Lehtinen, R Karjalainen, D Skinner, P Tooley, S Leong, A Ellingboe

Affiliation

¹ Department of Plant Pathology, University of Wisconsin, Madison 53706.

PMID: 2357737
DOI: 10.1007/BF00334519

Abstract

Low frequency, integrative transformation of three fertile hermaphroditic strains of Magnaporthe grisea has been achieved using plasmid pAN7-1 and cosmid pAN7-2, which contain an Escherichia coli hygromycin B phosphotransferase gene linked to Aspergillus nidulans regulatory sequences.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Ascomycota / drug effects
Ascomycota / genetics*
Blotting, Southern
Cosmids
Drug Resistance, Microbial / genetics
Hygromycin B / pharmacology*
Plasmids
Transformation, Genetic*

Substances

Anti-Bacterial Agents
Hygromycin B