In vitro activity of doripenem alone and in multi-agent combinations against extensively drug-resistant Acinetobacter baumannii and Klebsiella pneumoniae

Diagn Microbiol Infect Dis. 2013 Jul;76(3):343-6. doi: 10.1016/j.diagmicrobio.2013.03.014. Epub 2013 Apr 17.

Abstract

Carbapenems are increasingly needed to treat infections caused by drug-resistant gram-negative bacilli (GNB), but carbapenem resistance is increasing. We evaluated the activity of doripenem by broth microdilution against 96 extensively drug-resistant (XDR) Acinetobacter baumannii and Klebsiella pneumoniae isolates from patients with hospital-associated infections. All isolates were non-susceptible to doripenem, but ≥ 1 doripenem combination demonstrated synergy (fractional inhibitory concentration index: ≤ 0.5 for 2 agents, ≤ 0.75 for 3 agents) against 7 (15%) A. baumannii and 23 (48%) K. pneumoniae isolates; doripenem with rifampin and/or polymyxin B were most active. As doripenem has unique potential for use in prolonged infusions, suggested pharmacodynamic (PD) breakpoints range from 2-8 μg/mL; synergistic activity was found for higher proportions of XDR-GNB at higher PD breakpoints with doripenem with amikacin or with rifampin. The clinical utility of these observations requires further study, as treatment options for XDR-GNB infections are limited.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter Infections / microbiology
  • Acinetobacter baumannii / drug effects*
  • Amikacin / pharmacology
  • Anti-Bacterial Agents / pharmacology*
  • Carbapenems / pharmacology*
  • Cross Infection / microbiology
  • Doripenem
  • Drug Resistance, Multiple, Bacterial*
  • Drug Synergism
  • Humans
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / drug effects*
  • Microbial Sensitivity Tests
  • Polymyxin B / pharmacology
  • Rifampin / pharmacology

Substances

  • Anti-Bacterial Agents
  • Carbapenems
  • Amikacin
  • Doripenem
  • Polymyxin B
  • Rifampin