On-chip cytometry using plasmonic nanoparticle enhanced lensfree holography

Sci Rep. 2013:3:1699. doi: 10.1038/srep01699.

Abstract

Computational microscopy tools, in particular lensfree on-chip imaging, provide a large field-of-view along with a long depth-of-field, which makes it feasible to rapidly analyze large volumes of specimen using a compact and light-weight on-chip imaging architecture. To bring molecular specificity to this high-throughput platform, here we demonstrate the use of plasmon-resonant metallic nanoparticles to automatically recognize different cell types based on their plasmon-enhanced lensfree holograms, detected and reconstructed over a large field-of-view of e.g., ~24 mm².

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Equipment Design
  • Equipment Failure Analysis
  • Flow Cytometry / instrumentation*
  • Holography / instrumentation*
  • Imaging, Three-Dimensional / instrumentation*
  • Lenses
  • Microscopy / instrumentation*
  • Nanoparticles* / chemistry
  • Surface Plasmon Resonance / instrumentation*