Specific detection by real-time reverse-transcription PCR assays of a novel avian influenza A(H7N9) strain associated with human spillover infections in China

V M Corman; M Eickmann; O Landt; T Bleicker; S Brünink; M Eschbach-Bludau; M Matrosovich; S Becker; C Drosten

Specific detection by real-time reverse-transcription PCR assays of a novel avian influenza A(H7N9) strain associated with human spillover infections in China

Euro Surveill. 2013 Apr 18;18(16):20461.

Authors

V M Corman¹, M Eickmann, O Landt, T Bleicker, S Brünink, M Eschbach-Bludau, M Matrosovich, S Becker, C Drosten

Affiliation

¹ Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.

PMID: 23611031

Abstract

In response to a recent outbreak in China, detection assays for a novel avian influenza A(H7N9) virus need to be implemented in a large number of public health laboratories. Here we present real-time reverse-transcription polymerase chain reaction (RT-PCR) assays for specific detection of this virus, along with clinical validation data and biologically-safe positive controls.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Birds / virology
China
Humans
Influenza A virus / genetics*
Influenza A virus / isolation & purification
Influenza in Birds / transmission
Influenza in Birds / virology*
Influenza, Human / diagnosis
Influenza, Human / virology*
Real-Time Polymerase Chain Reaction / methods*