Abstract
Combined cancer treatment via co-delivery of siRNAs and an anticancer drug can be a promising strategy due to the synergistic effect of simultaneously minimizing gene/drug administration. In this study, Bcl-xL siRNA and doxorubicin (DOX) are encapsulated into designed methoxy-poly(ethylene glycol)-block-poly(D,L-lactic acid) (mPEG-b-PLA) block copolymer polymersomes (PSomes). A study of the cytotoxicity of Bcl-xL siRNA and DOX co-encapsulated PSomes (CPSomes) shows more inhibited proliferation of MKN-45 and MKN-28 human gastric cancer cell lines than only gene- and drug-loaded ones. Consequently, these results demonstrate that co-delivery of genes and drugs using PSomes results in a synergistic efficacy and indicates the potential of PSomes as efficient nanocarriers for combined cancer therapy.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / pharmacology*
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Apoptosis / drug effects
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Biocompatible Materials / chemistry*
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Cell Line, Tumor
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Cell Survival / drug effects
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Cryoelectron Microscopy
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Doxorubicin / pharmacology*
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Drug Delivery Systems*
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Gene Expression Regulation, Neoplastic / drug effects
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Gene Silencing / drug effects
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Humans
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Hydrogen-Ion Concentration / drug effects
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Hydrolysis / drug effects
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Inhibitory Concentration 50
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Kinetics
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Polyesters / chemistry*
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Polyethylene Glycols / chemistry*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Small Interfering / metabolism*
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Time Factors
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bcl-2-Associated X Protein / genetics
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bcl-2-Associated X Protein / metabolism
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bcl-X Protein / genetics
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bcl-X Protein / metabolism*
Substances
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Antineoplastic Agents
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Biocompatible Materials
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Polyesters
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RNA, Messenger
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RNA, Small Interfering
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bcl-2-Associated X Protein
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bcl-X Protein
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methoxy poly(ethylene glycol)-poly(lactide)
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Polyethylene Glycols
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Doxorubicin