Using high-throughput in situ plate screening to evaluate the effect of dehydration on protein crystals

Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):920-3. doi: 10.1107/S0907444913002412. Epub 2013 Apr 19.

Abstract

Crystal dehydration is a post-crystallization technique that can potentially improve the diffraction of macromolecular crystals. There are currently several ways of undertaking this process; however, dehydration experiments are often limited in their throughput and require prior manipulation of the samples. In the present study, a novel method is proposed that uses in situ plate screening to assess the effect of dehydration by combining the throughput of 96-well crystallization plates with direct X-ray feedback on crystal diffraction quality.

Keywords: dehydration; high throughput; in situ plate screening.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallization / instrumentation
  • Crystallization / methods
  • Crystallography, X-Ray
  • High-Throughput Screening Assays / methods*
  • Proteins / chemistry*

Substances

  • Proteins