Copper toxicity in Chinese cabbage is not influenced by plant sulphur status, but affects sulphur metabolism-related gene expression and the suggested regulatory metabolites

Plant Biol (Stuttg). 2014 Jan;16(1):68-78. doi: 10.1111/plb.12019. Epub 2013 May 3.

Abstract

The toxicity of high copper (Cu) concentrations in the root environment of Chinese cabbage (Brassica pekinensis) was little influenced by the sulphur nutritional status of the plant. However, Cu toxicity removed the correlation between sulphur metabolism-related gene expression and the suggested regulatory metabolites. At high tissue Cu levels, there was no relation between sulphur metabolite levels viz. total sulphur, sulphate and water-soluble non-protein thiols, and the expression and activity of sulphate transporters and expression of APS reductase under sulphate-sufficient or-deprived conditions, in the presence or absence of H2 S. This indicated that the regulatory signal transduction pathway of sulphate transporters was overruled or by-passed upon exposure to elevated Cu concentrations.

Keywords: APS reductase; Abiotic stress; H 2 S; heavy metals; sulphate deprivation; sulphate reduction; sulphate transporters; sulphur assimilation; thiol compounds.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brassica / genetics
  • Brassica / growth & development
  • Brassica / metabolism*
  • Copper / toxicity*
  • Gene Expression Regulation, Plant*
  • Genes, Plant
  • Hydrogen Sulfide / pharmacology
  • Sulfur / metabolism*

Substances

  • Sulfur
  • Copper
  • Hydrogen Sulfide