Abstract
Medulloblastoma is the most frequent type of embryonal tumour in the paediatric population. The disease progression in patients with this tumour may be connected with the presence of stem/tumour-initiating cells, but the precise source and characteristics of such cells is still a subject of debate. Thus, we tried to analyse biomarkers for which a connection with the presence of stem/tumour-initiating cells was suggested. We evaluated the transcriptional level of the ATOH1, FUT4, NGFR, OTX1, OTX2, PROM1 and SOX1 genes in 48 samples of medulloblastoma and analysed their usefulness in the prediction of disease outcome. The analyses showed a strong correlation of PROM1, ATOH1 and OTX1 gene expression levels with the outcome (p ≤ 0.2). On the basis of the multivariate Cox regression analysis, we propose a three-gene model predicting risk of the disease, calculated as follows: RS(risk score) =( 0:81 x PROM1) + (0:18 x OTX1) + (0:02 x ATOH1). Survival analysis revealed a better outcome among standard-risk patients, with a 5-year survival rate of 65 %, compared to the 40 % rate observed among high-risk patients. The most promising advantage of such molecular analysis consists in the identification of molecular markers influencing clinical behaviour, which may in turn be useful in therapy optimization.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
AC133 Antigen
-
Adolescent
-
Antigens, CD / genetics
-
Antigens, CD / metabolism*
-
Basic Helix-Loop-Helix Transcription Factors / genetics
-
Basic Helix-Loop-Helix Transcription Factors / metabolism*
-
Child
-
Child, Preschool
-
Female
-
Fucosyltransferases / genetics
-
Fucosyltransferases / metabolism
-
Glycoproteins / genetics
-
Glycoproteins / metabolism*
-
Humans
-
Infant
-
Lewis X Antigen / genetics
-
Lewis X Antigen / metabolism
-
Male
-
Medulloblastoma / diagnosis
-
Medulloblastoma / genetics*
-
Medulloblastoma / metabolism
-
Neoplasms, Germ Cell and Embryonal / diagnosis
-
Neoplasms, Germ Cell and Embryonal / genetics*
-
Neoplasms, Germ Cell and Embryonal / metabolism
-
Neoplastic Stem Cells / metabolism
-
Nerve Tissue Proteins / genetics
-
Nerve Tissue Proteins / metabolism
-
Otx Transcription Factors / genetics
-
Otx Transcription Factors / metabolism*
-
Peptides / genetics
-
Peptides / metabolism*
-
Prognosis
-
Receptors, Nerve Growth Factor / genetics
-
Receptors, Nerve Growth Factor / metabolism
-
SOXB1 Transcription Factors / genetics
-
SOXB1 Transcription Factors / metabolism
-
Transcription, Genetic*
Substances
-
AC133 Antigen
-
ATOH1 protein, human
-
Antigens, CD
-
Basic Helix-Loop-Helix Transcription Factors
-
Glycoproteins
-
Lewis X Antigen
-
NGFR protein, human
-
Nerve Tissue Proteins
-
OTX1 protein, human
-
OTX2 protein, human
-
Otx Transcription Factors
-
PROM1 protein, human
-
Peptides
-
Receptors, Nerve Growth Factor
-
SOX1 protein, human
-
SOXB1 Transcription Factors
-
FUT4 protein, human
-
Fucosyltransferases