Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers

Chen Wu; Yuanyuan Xie; Feng Gao; Yanan Wang; Yawei Guo; Huanna Tian; Yanshuang Li; Wufang Fan

doi:10.1016/j.gene.2013.04.067

Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers

Gene. 2013 Aug 1;525(1):18-25. doi: 10.1016/j.gene.2013.04.067. Epub 2013 May 9.

Authors

Chen Wu¹, Yuanyuan Xie, Feng Gao, Yanan Wang, Yawei Guo, Huanna Tian, Yanshuang Li, Wufang Fan

Affiliation

¹ College of Life Sciences, Hebei University, Baoding, Hebei 071002, PR China.

PMID: 23664892
DOI: 10.1016/j.gene.2013.04.067

Abstract

To explore Lgr5 as the possible stem cell marker in human gastric tissue, 259 normal gastric tissues and dissected gastric adenocarcinoma were analyzed by immunohistochemistry, immunofluorescence double staining and qRT-PCR. The results demonstrated that Lgr5 was expressed in the bottom of the normal gastric gland units, and showed a differential expression in gastric adenocarcinoma with varying differentiation. Lgr5 and Bmi1 were co-expressed within the same cells of gastric glands. CD26+, CD44+, ALDH1+ and CD133+ cells co-existed with Lgr5+ cells in the stem cell zone of adjacent normal gastric mucosa, and they were detectable in gastric adenocarcinoma but behaved differently. We concluded that Lgr5 may be the adult stem cell marker in human gastric epithelium; Lgr5 and Bmi1 may belong to the same stem cell population; Lgr5, CD26, CD44, ALDH1, and CD133 may be functionally-associated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma / genetics
Adenocarcinoma / metabolism
Adenocarcinoma / pathology
Aldehyde Dehydrogenase 1 Family
Antigens, CD / genetics
Antigens, CD / metabolism
Biomarkers, Tumor / biosynthesis*
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism
Cell Differentiation / genetics
Epithelium / metabolism
Gastric Mucosa / metabolism*
Humans
Isoenzymes / genetics
Isoenzymes / metabolism
Neoplastic Stem Cells / cytology
Neoplastic Stem Cells / metabolism*
Polycomb Repressive Complex 1 / genetics
Polycomb Repressive Complex 1 / metabolism
Receptors, G-Protein-Coupled / biosynthesis*
Receptors, G-Protein-Coupled / genetics
Receptors, G-Protein-Coupled / metabolism
Retinal Dehydrogenase / genetics
Retinal Dehydrogenase / metabolism
Retrospective Studies
Stem Cells / cytology
Stem Cells / metabolism*
Stomach Neoplasms / genetics
Stomach Neoplasms / metabolism
Stomach Neoplasms / pathology*

Substances

Antigens, CD
BMI1 protein, human
Biomarkers, Tumor
Isoenzymes
LGR5 protein, human
Receptors, G-Protein-Coupled
Aldehyde Dehydrogenase 1 Family
ALDH1A1 protein, human
Retinal Dehydrogenase
Polycomb Repressive Complex 1