Abstract
A population of Xiphinema macroacanthum Lamberti, Roca & Agostinelli, 1989 originating from olive orchards in Brindisi, Italy and containing both adults and all juvenile stages, is described and illustrated. The first juvenile stage is reported for the first time. Molecular characterisation of this species, using the D2-D3 expansion domains of the 28S rDNA and ITS region, was carried out. PCR-RFLP analyses of the ribosomal gene regions determined species-specific patterns that clearly differentiate X. macroacanthum. Sequences of the D2-D3 domains and the partial 18S-ITS1 rRNA genes were analysed using several methods for inferring phylogeny to reconstruct the relationships between X. macroacanthum and other Xiphinema species.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cluster Analysis
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DNA, Helminth / chemistry
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DNA, Helminth / genetics
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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DNA, Ribosomal Spacer / chemistry
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DNA, Ribosomal Spacer / genetics
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Genotype
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Italy
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Microscopy
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Molecular Sequence Data
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Nematoda / anatomy & histology
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Nematoda / classification*
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Nematoda / genetics
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Nematoda / isolation & purification*
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Olea / parasitology*
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Phylogeny
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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RNA, Ribosomal, 18S / genetics
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RNA, Ribosomal, 28S / genetics
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Sequence Analysis, DNA
Substances
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DNA, Helminth
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DNA, Ribosomal
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DNA, Ribosomal Spacer
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RNA, Ribosomal, 18S
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RNA, Ribosomal, 28S
Associated data
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GENBANK/HF546077
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GENBANK/HF546078
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GENBANK/HF546079
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GENBANK/HF546080
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GENBANK/HF546081