Abstract
The transmission of T-2 toxin and its metabolites into the edible tissues of poultry has potential effects on human health. We report that T-2 toxin significantly induces CYP1A4 and CYP1A5 expression in chicken embryonic hepatocyte cells. The enzyme activity assays of CYP1A4 and CYP1A5 heterologously expressed in HeLa cells indicate that only CYP1A5 metabolizes T-2 to 3'OH-T-2 by the 3'-hydroxylation of isovaleryl groups. In vitro enzyme assays of recombinant CYP1A5 expressed in DH5α further confirm that CYP1A5 can convert T-2 into TC-1 (3'OH-T-2). Therefore, CYP1A5 is critical for the metabolism of trichothecene mycotoxin in chickens.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Aryl Hydrocarbon Hydroxylases / genetics
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Aryl Hydrocarbon Hydroxylases / metabolism*
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Biocatalysis
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Chickens / metabolism*
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Chromatography, Liquid
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HeLa Cells
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Humans
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Inactivation, Metabolic*
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Protein Structure, Secondary
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Real-Time Polymerase Chain Reaction
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Recombinant Proteins / chemistry
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Recombinant Proteins / isolation & purification
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Spectrometry, Mass, Electrospray Ionization
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T-2 Toxin / analogs & derivatives*
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T-2 Toxin / chemistry
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T-2 Toxin / metabolism
Substances
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Recombinant Proteins
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3'-hydroxy-T-2 toxin
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Aryl Hydrocarbon Hydroxylases
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cytochrome P-450 CYP1A5
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T-2 Toxin