Interaction between calcineurin inhibitors and IL-28B rs12979860 C>T polymorphism and response to treatment for post-transplant recurrent hepatitis C

Dig Liver Dis. 2013 Nov;45(11):927-32. doi: 10.1016/j.dld.2013.04.006. Epub 2013 May 27.

Abstract

Background: The impact of calcineurin inhibitors on achievement of sustained virological response to antiviral therapy for post-transplant recurrent hepatitis C is controversial. This study aimed at investigating the interactions between calcineurin inhibitors and interleukin-28B (IL-28B) gene polymorphisms and sustained virological response.

Methods: Retrospective study of 147 liver transplant recipients with recurrent hepatitis C, who received 48 weeks of peg-interferon-α (N=113) or standard interferon (N=34), in association with ribavirin. Cyclosporine and tacrolimus were administered in 68 and 79 patients, respectively. IL-28B rs12979860 allele frequency was assessed in both donors and recipients.

Results: Overall, 57 patients (38.8%) obtained sustained virological response; no difference was found between cyclosporine and tacrolimus-treated patients (42.6% vs. 35.4%, p=0.371). Recipient and donor IL-28B genotypic frequencies were C/C=30.6%, C/T=51.7%, T/T=17.7% and C/C=44.9%, C/T=50.3%, T/T=4.8%, respectively. Combining donor and recipient alleles, response rates decreased from cyclosporine-treated patients carrying ≤ 1 T allele (56.1%) to tacrolimus-treated patients carrying ≤ 1 T allele (44.7%) to patients carrying ≥ 2 T alleles (25.0%, p=0.0009).

Conclusions: Donor and recipient rs12979860 alleles synergistically influence sustained virological response rate to antiviral treatment for recurrent hepatitis C. In patients carrying <2 T alleles cyclosporine favours a better response than tacrolimus, while no difference was found in the presence of ≥ 2 T alleles.

Keywords: Antiviral therapy; Calcineurin inhibitors; Chronic hepatitis; Interleukin-28B polymorphisms; Liver transplantation.

Publication types

  • Multicenter Study

MeSH terms

  • Alleles
  • Antiviral Agents / therapeutic use
  • Calcineurin Inhibitors*
  • DNA / genetics*
  • Drug Therapy, Combination
  • Follow-Up Studies
  • Genotype
  • Hepacivirus / drug effects
  • Hepacivirus / genetics
  • Hepatitis C, Chronic / etiology
  • Hepatitis C, Chronic / genetics
  • Hepatitis C, Chronic / therapy*
  • Interferon-alpha / therapeutic use*
  • Interferons
  • Interleukins / genetics*
  • Interleukins / metabolism
  • Liver Transplantation / adverse effects*
  • Polyethylene Glycols / therapeutic use*
  • Polymorphism, Genetic*
  • RNA, Viral / genetics
  • Recombinant Proteins / therapeutic use
  • Recurrence
  • Retrospective Studies
  • Ribavirin / therapeutic use
  • Treatment Outcome
  • Viral Load / drug effects

Substances

  • Antiviral Agents
  • Calcineurin Inhibitors
  • interferon-lambda, human
  • Interferon-alpha
  • Interleukins
  • RNA, Viral
  • Recombinant Proteins
  • Polyethylene Glycols
  • Ribavirin
  • DNA
  • Interferons
  • peginterferon alfa-2a