A mutation in the stalk of the newcastle disease virus hemagglutinin-neuraminidase (HN) protein prevents triggering of the F protein despite allowing efficient HN-F complex formation

J Virol. 2013 Aug;87(15):8813-5. doi: 10.1128/JVI.01066-13. Epub 2013 Jun 5.

Abstract

Newcastle disease virus (NDV)-induced membrane fusion requires formation of a complex between the hemagglutinin-neuraminidase (HN) and fusion (F) proteins. Substitutions for NDV HN stalk residues A89, L90, and L94 block fusion by modulating formation of the HN-F complex. Here, we demonstrate that a nearby L97A substitution, though previously shown to block fusion, allows efficient HN-F complex formation and likely acts by preventing changes in the HN stalk required for triggering of the bound F protein.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Substitution
  • HN Protein / genetics
  • HN Protein / metabolism*
  • Mutant Proteins / genetics
  • Mutant Proteins / metabolism
  • Newcastle disease virus / genetics
  • Newcastle disease virus / physiology*
  • Protein Binding
  • Protein Multimerization*
  • Viral Fusion Proteins / metabolism*
  • Virus Internalization*

Substances

  • HN Protein
  • Mutant Proteins
  • Viral Fusion Proteins