Ciliated micropillars for the microfluidic-based isolation of nanoscale lipid vesicles

Lab Chip. 2013 Aug 7;13(15):2879-82. doi: 10.1039/c3lc41343h.

Abstract

We fabricated a microfluidic device consisting of ciliated micropillars, forming a porous silicon nanowire-on-micropillar structure. We demonstrated that the prototype device can preferentially trap exosome-like lipid vesicles, while simultaneously filtering out proteins and cell debris. Trapped lipid vesicles can be recovered intact by dissolving the porous nanowires in PBS buffer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Equipment Design
  • Filtration / instrumentation
  • Lipids / isolation & purification*
  • Microfluidic Analytical Techniques / instrumentation*
  • Nanowires / chemistry
  • Porosity
  • Silicon / chemistry

Substances

  • Lipids
  • Silicon