Whole-genome sequence analysis of a Korean G11P[25] rotavirus strain identifies several porcine-human reassortant events

Arch Virol. 2013 Nov;158(11):2385-93. doi: 10.1007/s00705-013-1720-9. Epub 2013 Jun 7.

Abstract

A rare rotavirus, RVA/Human-wt/KOR/CAU12-2/2012/G11P[25], was isolated from a 16-year-old female with fever and diarrhea during the 2012 rotavirus surveillance in South Korea using a cell culture system, and its full genome sequence was determined and analyzed. Strain CAU12-2 exhibited a G11-P[25]-I12-R1-C1-M1-A1-N1-T1-E1-H1 genotype constellation. Phylogenetic analysis of this strain revealed that it is a human-porcine reassortant of two distant relatives of the G11 strains circulating in the world. The VP7 and VP4 genes are most closely related to those of human G11P[25] viruses (Dhaka6, KTM368, and N-38 strains) identified in South Asia, whereas the VP1 gene originated from a porcine G11P[7] virus (YM strain) that was identified in South America. The VP6 gene was found to belong to the new genotype I12. This study indicates that the G11-P[25]-I12 genotype was introduced into the South Korean population by interspecies transmissions of human and animal rotaviruses, followed by multiple reassortment events.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Diarrhea / virology
  • Genome, Viral*
  • Genotype
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Viral / genetics
  • Reassortant Viruses / genetics*
  • Republic of Korea
  • Rotavirus / classification
  • Rotavirus / genetics*
  • Rotavirus / isolation & purification
  • Rotavirus Infections / epidemiology
  • Rotavirus Infections / virology*
  • Sequence Analysis, DNA
  • Swine / virology*
  • Viral Proteins / genetics

Substances

  • RNA, Viral
  • Viral Proteins

Associated data

  • GENBANK/KC140587
  • GENBANK/KC140588
  • GENBANK/KC140589
  • GENBANK/KC140590
  • GENBANK/KC140591
  • GENBANK/KC140592
  • GENBANK/KC140593
  • GENBANK/KC140594
  • GENBANK/KC140595
  • GENBANK/KC140596
  • GENBANK/KC140597