Optimal conditions for directly sequencing double-stranded PCR products with sequenase

Nucleic Acids Res. 1990 Jul 11;18(13):4028. doi: 10.1093/nar/18.13.4028.
No abstract available

MeSH terms

  • Base Sequence*
  • DNA
  • DNA-Directed DNA Polymerase / metabolism*
  • Nucleic Acid Amplification Techniques*
  • Polymerase Chain Reaction / methods*
  • Temperature
  • Time Factors

Substances

  • DNA
  • bacteriophage T7 induced DNA polymerase
  • DNA-Directed DNA Polymerase