Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling

Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.

Authors

Zhengqiu Li¹, Piliang Hao, Lin Li, Chelsea Y J Tan, Xiamin Cheng, Grace Y J Chen, Siu Kwan Sze, Han-Ming Shen, Shao Q Yao

Affiliation

¹ Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.

PMID: 23754342
DOI: 10.1002/anie.201300683

No abstract available

Keywords: affinity-based probes; fluorescent probes; kinases; photo-crosslinking; proteomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkynes / chemistry*
Cell Proliferation / drug effects
Cross-Linking Reagents
Diazomethane / chemistry*
Drug Design*
Fluorescent Antibody Technique
Hep G2 Cells
Humans
Inhibitory Concentration 50
Kinetics
Molecular Probes / chemistry*
Molecular Structure
Photoaffinity Labels / chemistry
Protein Kinase Inhibitors / chemical synthesis*
Protein Kinase Inhibitors / pharmacology
Protein Kinases / chemistry*
Protein Kinases / metabolism
Proteome / analysis*
Proteomics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

Alkynes
Cross-Linking Reagents
Molecular Probes
Photoaffinity Labels
Protein Kinase Inhibitors
Proteome
Diazomethane
Protein Kinases