Rim15p-mediated regulation of sucrose utilization during molasses fermentation using Saccharomyces cerevisiae strain PE-2

J Biosci Bioeng. 2013 Nov;116(5):591-4. doi: 10.1016/j.jbiosc.2013.05.015. Epub 2013 Jun 10.

Abstract

Inherited loss-of-function mutations in the Rim15p-mediated stress-response pathway contribute to the high fermentation rate of sake yeast strains. In the present study, we found that disruption of the RIM15 gene in ethanol-producing Saccharomyces cerevisiae strain PE-2 accelerated molasses fermentation through enhanced sucrose utilization following glucose starvation.

Keywords: Alcoholic fermentation; Msn2p; Rim15p; Saccharomyces cerevisiae; Sake yeast; Stress response; Sucrose utilization; Sugarcane molasses.

MeSH terms

  • Alcoholic Beverages / microbiology
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Ethanol / metabolism
  • Fermentation*
  • Glucose / deficiency
  • Glucose / metabolism
  • Molasses / microbiology*
  • Protein Kinases / deficiency
  • Protein Kinases / genetics
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Stress, Physiological
  • Sucrose / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • DNA-Binding Proteins
  • MSN2 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Ethanol
  • Sucrose
  • Protein Kinases
  • Rim15 protein, S cerevisiae
  • Glucose