B cell translocation gene, a direct target of miR-142-5p, inhibits vascular smooth muscle cell proliferation by down-regulating cell cycle progression

FEBS Lett. 2013 Aug 2;587(15):2385-92. doi: 10.1016/j.febslet.2013.06.005. Epub 2013 Jun 13.

Abstract

Vascular smooth muscle cell (VSMC) proliferation plays a key role in neointimal hyperplasia and restenosis. Here we report the role of the microRNA miR-142-5p and its downstream target genes on the proliferation of cultured VSMCs. miR-142-5p promoted VSMC proliferation by down-regulating B cell translocation gene 3 (BTG3). We found that BTG3 inhibited the expression of cell cycle regulatory genes and cell growth. As shown by luciferase reporter assay, miR-142-5p bound directly to the 3'-untranslated region of BTG3. Overexpression of miR-142-5p induced expression of cell cycle regulatory genes. Thus, BTG3, a novel, direct target of miR-142-5p, negatively regulates VSMC proliferation.

Keywords: BTG3; Neointima hyperplasia; Proliferation; Vascular smooth muscle cell; miR-142-5p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle / physiology*
  • Cell Proliferation*
  • Cells, Cultured
  • Down-Regulation*
  • Male
  • MicroRNAs / genetics*
  • Muscle, Smooth, Vascular / cytology*
  • Proteins / genetics
  • Proteins / physiology*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Btg3 protein, rat
  • MIRN142 microRNA, rat
  • MicroRNAs
  • Proteins