Nonenzymatic labeling of 5-hydroxymethylcytosine in nanopore sequencing

Xingyu Lu; Chuan He

doi:10.1002/cbic.201300342

Nonenzymatic labeling of 5-hydroxymethylcytosine in nanopore sequencing

Chembiochem. 2013 Jul 22;14(11):1289-90. doi: 10.1002/cbic.201300342. Epub 2013 Jun 18.

Authors

Xingyu Lu¹, Chuan He

Affiliation

¹ Department of Chemistry, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA.

PMID: 23780928
DOI: 10.1002/cbic.201300342

Abstract

Labeling chemically for a "single" result: A one-step, nonenzymatic 5hmC thiolation method mediated by bisulfite is highlighted. The 5hmC base can be chemically labeled in single-stranded DNA and detected by using nanopore sequencing.

Keywords: 5-hydroxylmethylcytosine; DNA methylation; epigenetics; nanopores; nonenzymatic labeling.

MeSH terms

5-Methylcytosine / analogs & derivatives
Cytosine / analogs & derivatives*
Cytosine / chemistry
DNA Methylation
DNA, Single-Stranded / chemistry*
DNA, Single-Stranded / metabolism
Nanopores*
Sequence Analysis, DNA

Substances

DNA, Single-Stranded
5-hydroxymethylcytosine
5-Methylcytosine
Cytosine