Impact of dye-protein interaction and silver nanoparticles on rose bengal photophysical behavior and protein photocrosslinking

Photochem Photobiol. 2013 Nov-Dec;89(6):1433-41. doi: 10.1111/php.12119. Epub 2013 Aug 17.

Abstract

The role of recombinant Type-I human collagen in the free form or forming AgNP@collagen on the photophysical and photochemical behavior of rose Bengal was analyzed. The formation of dye aggregates on the protein surface was experimentally observed and corroborated by docking calculations. The formation of such aggregates is believed to change the main oxidative mechanism from Type-II (singlet oxygen) to Type-I (free radical) photosensitization. Remarkably, the presence of AgNP in the form of AgNP@collagen altered the dynamics of dye triplet deactivation, effectively preventing the dye degradation and reducing the extent of protein crosslinked. Both crosslinked rHC and AgNP@collagen were able to support fibroblasts proliferation, but only the material containing silver was resistant to S. epidermidis infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cross-Linking Reagents / chemistry
  • Metal Nanoparticles*
  • Photochemistry
  • Rose Bengal / chemistry*
  • Silver / chemistry*

Substances

  • Cross-Linking Reagents
  • Rose Bengal
  • Silver