[Effect of BCL11A gene on transcription of γ-globin gene]

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2013 Jun;21(3):628-32. doi: 10.7534/j.issn.1009-2137.2013.03.018.
[Article in Chinese]

Abstract

This study was aimed to explore the effect of BCL11A gene on transcription of γ-globin gene in K562 cells. B-cell lymphoma/leukemia 11A (BCL11A) gene was silenced by small interfering RNA (siRNA) expression vectors in K562 cells (human erythroblastic leukemia cell line). Gamma-globin mRNA level in K562 cells was determined by RT-PCR. Association between the BCL11A gene and γ-globin gene transcription was explored by comparison of mRNA levels. The results indicated that the silence rate of the BCL11A gene in K562 cells by 4 siRNA expression vectors was 49.7%, 55.4%, 78.2%, and 84.1%, respectively. The siRNA expression vector with 84.1% silence rate was transfected into K562 cells, transcription level of γ-globin mRNA in K562 cells transfected with siRNA expression vector increased 2.4 times as compared with control K562 cells. It is concluded that level of γ-globin mRNA increases when the BCL11A gene is silenced. It indicates that the BCL11A gene may be a negative regulator for γ-globin gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / genetics*
  • Gene Expression Regulation, Leukemic
  • Genes, Regulator
  • Genetic Vectors
  • Humans
  • K562 Cells
  • Nuclear Proteins / genetics*
  • RNA Interference
  • RNA, Small Interfering / genetics
  • Repressor Proteins
  • Transcription, Genetic*
  • Transfection
  • gamma-Globins / genetics*

Substances

  • BCL11A protein, human
  • Carrier Proteins
  • Nuclear Proteins
  • RNA, Small Interfering
  • Repressor Proteins
  • gamma-Globins