Rapid assay for detecting gyrA and parC mutations associated with fluoroquinolone resistance in Enterobacteriaceae

Ryuichi Nakano; Ryoichi Okamoto; Akiyo Nakano; Noriyuki Nagano; Michiko Abe; Shigeru Tansho-Nagakawa; Tsuneyuki Ubagai; Takane Kikuchi-Ueda; Osamu Koshio; Hirotoshi Kikuchi; Yasuo Ono

doi:10.1016/j.mimet.2013.06.019

Rapid assay for detecting gyrA and parC mutations associated with fluoroquinolone resistance in Enterobacteriaceae

J Microbiol Methods. 2013 Sep;94(3):213-6. doi: 10.1016/j.mimet.2013.06.019. Epub 2013 Jun 28.

Authors

Ryuichi Nakano¹, Ryoichi Okamoto, Akiyo Nakano, Noriyuki Nagano, Michiko Abe, Shigeru Tansho-Nagakawa, Tsuneyuki Ubagai, Takane Kikuchi-Ueda, Osamu Koshio, Hirotoshi Kikuchi, Yasuo Ono

Affiliation

¹ Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan. [email protected]

PMID: 23816531
DOI: 10.1016/j.mimet.2013.06.019

Abstract

We developed a PCR-RFLP assay to detect mutations in the quinolone-resistance determining regions of gyrA and parC associated with fluoroquinolone resistance in Enterobacteriaceae. The assay detected mutations associated with reduced susceptibility to fluoroquinolones and may therefore serve as a specific, rapid, inexpensive, and simple testing alternative.

Keywords: Enterobacteriaceae; Fluoroquinolones; PCR-restriction fragment length polymorphism; Quinolone resistance-determining regions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Substitution / genetics
Base Sequence
DNA Gyrase / genetics*
DNA Topoisomerase IV / genetics*
Drug Resistance, Bacterial / genetics*
Enterobacteriaceae* / drug effects
Enterobacteriaceae* / genetics
Fluoroquinolones / pharmacology*
Microbial Sensitivity Tests
Molecular Sequence Data
Mutation / genetics
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Sequence Alignment

Substances

Fluoroquinolones
DNA Topoisomerase IV
DNA Gyrase