A Low Cost Metal-Free Vascular Access Mini-Port for Artifact Free Imaging and Repeated Injections in Mice

PLoS One. 2013 Jun 18;8(6):e65939. doi: 10.1371/journal.pone.0065939. Print 2013.

Abstract

Purpose: Small injection ports for mice are increasingly used for drug testing or when administering contrast agents. Commercially available mini-ports are expensive single-use items that cause imaging-artifacts. We developed and tested an artifact-free, low-cost, vascular access mini-port (VAMP) for mice.

Procedures: Leakage testing of the VAMP was conducted with high speed bolus injections of different contrast agents. VAMP-induced artifacts were assessed using a micro-CT and a small animal MRI (9.4T) scanner ex vivo. Repeated contrast administration was performed in vivo.

Results: With the VAMP there was no evidence of leakage with repeated punctures, high speed bolus contrast injections, and drawing of blood samples. In contrast to the tested commercially available ports, the VAMP did not cause artifacts with MRI or CT imaging.

Conclusions: The VAMP is an alternative to commercially available mini-ports and has useful applications in animal research involving imaging procedures and contrast agent testing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Artifacts*
  • Mice
  • Vascular Access Devices*
  • Vascular Surgical Procedures
  • X-Ray Microtomography

Grants and funding

The acquisition of the micro-CT (Yxlon Y. Fox) was funded by the Federal Ministry of Education and Research and the Land Baden- Württemberg (HBFG grant # 125-648). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.