The BALB/c mouse as a model for immunological studies of microfilariae-induced pulmonary eosinophilia

Am J Trop Med Hyg. 1990 Jul;43(1):61-6. doi: 10.4269/ajtmh.1990.43.61.

Abstract

Tropical pulmonary eosinophilia (TPE) is believed to result from extreme immediate hypersensitivity to microfilariae localized in the pulmonary vasculature of some persons with lymphatic filariasis. Female BALB/c mice repeatedly immunized by ip injection of Brugia malayi microfilariae become amicrofilaremic within 24 hr of iv parasite challenge, whereas non-sensitized control animals remain patent for greater than 72 hr. Immunized, but not control mice, develop peripheral blood and pulmonary eosinophilia (2,000 cells/mm3 and 65,000 cells/bronchoalveolar lavage, respectively). Serum and bronchoalveolar lavage filarial-specific IgG antibodies are greater in sensitized mice than in controls (ELISA absorbance values 20- and 10-fold higher, respectively). Serum IgE antibody levels are also greater (P less than 0.01) in immunized parasite-challenged mice than in controls (mean cpm 125I-labeled anti-mouse IgE bound to B. malayi antigen-coated Sepharose beads: 7,852 vs. 1,741, respectively). This model exhibits several of the major features of human TPE: amicrofilaremia, elevated levels of serum IgG and IgE antibodies to microfilariae, and blood and pulmonary eosinophilia. This model may be useful in the examination of the role of filarial antigen-specific lymphoid cells and antibodies in regulating the pathologic responses to microfilariae trapped in the lung.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Helminth / biosynthesis*
  • Antibodies, Helminth / blood
  • Bronchoalveolar Lavage Fluid / immunology
  • Brugia / immunology*
  • Disease Models, Animal*
  • Female
  • Immunoglobulin E / biosynthesis
  • Immunoglobulin G / biosynthesis
  • Mice
  • Mice, Inbred BALB C*
  • Microfilariae / immunology
  • Pulmonary Eosinophilia / immunology*

Substances

  • Antibodies, Helminth
  • Immunoglobulin G
  • Immunoglobulin E