Complete sequence of pSAM7, an IncX4 plasmid carrying a novel blaCTX-M-14b transposition unit isolated from Escherichia coli and Enterobacter cloacae from cattle

Antimicrob Agents Chemother. 2013 Sep;57(9):4590-4. doi: 10.1128/AAC.01157-13. Epub 2013 Jul 8.

Abstract

The same plasmid carrying blaCTX-M-14b was identified from an Escherichia coli isolate and an Enterobacter cloacae isolate collected from cattle in the United Kingdom by complete plasmid sequencing. This 35,341-bp plasmid, pSAM7, had an IncX4 backbone that is 99% identical to that of pJIE143 from a human isolate in Australia. PCR screening identified pSAM7-like plasmids in three other E. coli isolates of different multilocus sequence types isolated from cattle on different farms in the United Kingdom.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Australia / epidemiology
  • Cattle
  • Cattle Diseases / epidemiology
  • Cattle Diseases / microbiology*
  • DNA Transposable Elements*
  • Enterobacter cloacae / enzymology
  • Enterobacter cloacae / genetics*
  • Enterobacteriaceae Infections / epidemiology
  • Enterobacteriaceae Infections / microbiology
  • Enterobacteriaceae Infections / veterinary*
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Humans
  • Multilocus Sequence Typing
  • Plasmids*
  • Sequence Analysis, DNA
  • United Kingdom / epidemiology
  • beta-Lactamases / chemistry*
  • beta-Lactamases / genetics
  • beta-Lactamases / isolation & purification

Substances

  • DNA Transposable Elements
  • beta-Lactamases