Abstract
The NADPH oxidase of human eosinophils, measured in the cell-free system, shows the same characteristics as the enzyme from human neutrophils. All proteins required for activity of the enzyme are expressed in eosinophils at a higher level than in neutrophils. Eosinophils isolated from patients with chronic granulomatous disease show the same molecular defects as the neutrophils from these patients.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / metabolism
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Blotting, Western
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Cell Membrane / enzymology
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Cell-Free System
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Enzyme Activation / drug effects
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Eosinophils / enzymology*
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Granulomatous Disease, Chronic / enzymology
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Humans
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NADH, NADPH Oxidoreductases / metabolism*
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NADPH Oxidases
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Neutrophils / enzymology
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Sodium Dodecyl Sulfate / pharmacology
Substances
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Sodium Dodecyl Sulfate
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NADH, NADPH Oxidoreductases
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NADPH Oxidases
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Alkaline Phosphatase