Abstract
The purpose of this study was to identify a mechanism related to miRNA pathway which plays a role in the anti-tumor effects of Diallyl disulfide. Alterations in miRNA expression were observed in Diallyl disulfide-treated MGC-803 cells, including up-regulation of miR-200b and miR-22 expression. Furthermore, Wnt-1 was identified as a target of both miR-200b and miR-22. MiR-200b and miR-22 not only synergistically inhibited gastric cancer growth, but also enhanced the antitumor effect of Diallyl disulfide both in vitro and in vivo. It indicated that miR-200b and miR-22 may serve as potential gene therapy and enhance Diallyl disulfide antitumor effects.
Keywords:
Diallyl disulfide; Gastric cancer; MicroRNA; Wnt-1 signaling pathway.
Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / pathology
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Adenocarcinoma / therapy*
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Allyl Compounds / pharmacology*
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Animals
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Antineoplastic Agents / pharmacology*
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Apoptosis / drug effects
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Base Sequence
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Combined Modality Therapy
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Disulfides / pharmacology*
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Gene Expression Regulation, Neoplastic
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Genetic Therapy
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Humans
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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MicroRNAs / genetics
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MicroRNAs / metabolism*
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RNA Interference
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Stomach Neoplasms / pathology
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Stomach Neoplasms / therapy*
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Transcriptome / drug effects
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Tumor Burden
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Up-Regulation
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Wnt Signaling Pathway
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Wnt1 Protein / genetics
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Wnt1 Protein / metabolism*
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Xenograft Model Antitumor Assays
Substances
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Allyl Compounds
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Antineoplastic Agents
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Disulfides
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MIRN200 microRNA, human
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MIRN22 microRNA, human
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MicroRNAs
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Wnt1 Protein
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diallyl disulfide