Sphingomicrobium marinum sp. nov. and Sphingomicrobium flavum sp. nov., isolated from surface seawater, and emended description of the genus Sphingomicrobium

Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4469-4476. doi: 10.1099/ijs.0.052837-0. Epub 2013 Jul 16.

Abstract

Two Gram-staining-negative, yellow-pigmented, rod-shaped, strictly aerobic, non-flagellated and non-spore-forming amylolytic marine bacterial strains, designated CC-AMZ-30M(T) and CC-AMZ-30N(T), were isolated from coastal surface seawater in Taiwan. Strain CC-AMZ-30M(T) shared pairwise 16S rRNA gene sequence similarities of 95.8, 95.0 and <94.0 % to Sphingomicrobium lutaoense CC-TBT-3(T), Sphingomicrobium astaxanthinifaciens CC-AMO-30B(T) and other sphingomonads, respectively. Strain CC-AMZ-30N(T) shared 97.0, 96.7, 95.0 and <95.1 % similarities to strain CC-AMZ-30M(T), Sphingomicrobium lutaoense CC-TBT-3(T), Sphingomicrobium astaxanthinifaciens CC-AMO-30B(T) and other sphingomonads, respectively. The common polar lipids of the two strains include a signature glycolipid (GL2), diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and sphingoglycolipid in major amounts besides moderate-to-trace amounts of an unidentified aminolipid and several unidentified glycolipids. Both strains contained C18 : 1ω7c/C18 : 1ω6c, C16 : 1ω7c/C16 : 1ω6c, C16 : 0 and C18 : 1 2-OH as major (>5 % of the total) fatty acids. Strains CC-AMZ-30M(T) and CC-AMZ-30N(T) had DNA G+C contents of 64.2 and 65.2 mol%, respectively. The major polyamine was spermidine in strain CC-AMZ-30M(T) and triamine sym-homospermidine in strain CC-AMZ-30N(T). Both strains contained ubiquinone Q-10 as the predominant respiratory quinone. Differential phylogenetic and chemotaxonomic evidence including the presence of characteristic GL2, C18 : 1 2-OH and several other phenotypic features supported the classification of strains CC-AMZ-30M(T) and CC-AMZ-30N(T) as two novel species of the genus Sphingomicrobium, for which we propose the names Sphingomicrobium marinum sp. nov. and Sphingomicrobium flavum sp. nov., respectively; corresponding type strains are Sphingomicrobium marinum CC-AMZ-30M(T) ( = JCM 18554(T) = BCRC 80466(T)) and Sphingomicrobium flavum CC-AMZ-30N(T) ( = JCM 18555(T) = BCRC 80467(T)). An emended description of the genus Sphingomicrobium is also proposed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Glycolipids / chemistry
  • Molecular Sequence Data
  • Phylogeny*
  • Polyamines / chemistry
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Sphingomonadaceae / classification*
  • Sphingomonadaceae / genetics
  • Sphingomonadaceae / isolation & purification
  • Taiwan
  • Ubiquinone / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Glycolipids
  • Polyamines
  • RNA, Ribosomal, 16S
  • Ubiquinone
  • Ubiquinone Q2

Associated data

  • GENBANK/JX235672
  • GENBANK/JX393854