Abstract
Mice of a transgenic mouse lineage 501, produced by zygotic injection of the parotid alpha-amylase promoter-SV40 T-antigen hybrid gene, developed osteosarcomas at about 15 months of age. The tumors predominantly involved the axial skeleton, were sometimes multiple, and metastasized to the liver. A cell line derived from a primary tumor produced osteosarcomas on transfer to nude mice. The 501 transgenic lineage thus provides a valuable new model for studying the histogenesis of osteosarcomas.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, Polyomavirus Transforming / genetics*
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Antigens, Polyomavirus Transforming / metabolism
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Bone Neoplasms / genetics*
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Bone Neoplasms / metabolism
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Bone Neoplasms / pathology
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Cell Line
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Cloning, Molecular
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Mice
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Mice, Transgenic / genetics*
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Osteosarcoma / genetics*
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Osteosarcoma / metabolism
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Osteosarcoma / pathology
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Promoter Regions, Genetic
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Tumor Cells, Cultured / metabolism
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Tumor Cells, Cultured / pathology
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alpha-Amylases / genetics*
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alpha-Amylases / metabolism
Substances
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Antigens, Polyomavirus Transforming
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alpha-Amylases