CdTe quantum dot as a fluorescence probe for vitamin B(12) in dosage form

Spectrochim Acta A Mol Biomol Spectrosc. 2013 Nov:115:603-9. doi: 10.1016/j.saa.2013.06.068. Epub 2013 Jul 1.

Abstract

We here report the CdTe quantum dot (CdTe QDs)-based sensor for probing vitamin B12 derivatives in aqueous solution. In this paper, simple and sensitive fluorescence quenching measurements has been employed. The Stern-Volmer constant (KSV), quenching rate constant (kq) and binding constant (K) were rationalized from fluorescence quenching measurement. Furthermore, the fluorescence resonance energy transfer (FRET) mechanism was discussed. This method was applicable over the concentration ranging from 1 to 14μg/mL (VB12) with correlation coefficient of 0.993. The limit of detection (LOD) of VB12 was found to be 0.15μg/mL. Moreover, the present approach opens a simple pathway for developing cost-effective, sensitive and selective QD-based fluorescence sensors/probes for biologically significant VB12 in pharmaceutical sample with mean recoveries in the range of 100-102.1%.

Keywords: CdTe quantum dot; FRET; Spectrofluorimetry; Vitamin B(12).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorption
  • Cadmium Compounds / chemistry*
  • Dosage Forms*
  • Electrochemical Techniques
  • Energy Transfer
  • Fluorescent Dyes / chemistry*
  • Humans
  • Hydrogen-Ion Concentration
  • Kinetics
  • Limit of Detection
  • Quantum Dots*
  • Spectrometry, Fluorescence
  • Spectrophotometry, Ultraviolet
  • Tellurium / chemistry*
  • Thiomalates / chemistry
  • Vitamin B 12 / analysis*
  • X-Ray Diffraction

Substances

  • Cadmium Compounds
  • Dosage Forms
  • Fluorescent Dyes
  • Thiomalates
  • 2-thiomalic acid
  • Tellurium
  • Vitamin B 12
  • cadmium telluride