Structural peculiarities of the (MHF1-MHF2)4 octamer provide a long DNA binding patch to anchor the MHF-FANCM complex to chromatin: a solution SAXS study

Wenjia Wang; Qiong Guo; Eleonora V Shtykova; Guangfeng Liu; Jianhua Xu; Maikun Teng; Peng Liu; Yuhui Dong

doi:10.1016/j.febslet.2013.07.022

Structural peculiarities of the (MHF1-MHF2)4 octamer provide a long DNA binding patch to anchor the MHF-FANCM complex to chromatin: a solution SAXS study

FEBS Lett. 2013 Sep 17;587(18):2912-7. doi: 10.1016/j.febslet.2013.07.022. Epub 2013 Jul 22.

Authors

Wenjia Wang¹, Qiong Guo, Eleonora V Shtykova, Guangfeng Liu, Jianhua Xu, Maikun Teng, Peng Liu, Yuhui Dong

Affiliation

¹ Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.

PMID: 23886707
DOI: 10.1016/j.febslet.2013.07.022

Abstract

MHF1 and MHF2 are histone-fold-containing FANCM-associated proteins. FANCM is a Fanconi anemia (FA) complementation group protein. We previously obtained high-resolution structures of MHF1-MHF2 (MHF) and MHF in complex with a fragment of FANCM (MHF-FANCM-F). Here, we use small angle X-ray scattering (SAXS) to investigate the solution behaviors of these protein complexes. In combination with crystallographic data, the results of the SAXS study reveal that a long, positively charged patch exposed on the surface of the MHF complex plays a critical role in double-stranded DNA (dsDNA) binding.

Keywords: DNA binding patch; FA; FANCM; Fanconi anaemia; Fanconi anemia; ICLs; MHF; MHF complex; MHF in complex with a fragment of FANCM; MHF1–MHF2; MHF–FANCM-F; MM; SAXS; SEC; double strand DNA; dsDNA; interstrand crosslinks; molecular mass; size-exclusion chromatography.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis Regulatory Proteins / chemistry*
Apoptosis Regulatory Proteins / genetics
Binding Sites
Chromatin / chemistry*
Chromatin / genetics
DNA / chemistry*
DNA / genetics
DNA Helicases / chemistry*
DNA Helicases / genetics
DNA-Binding Proteins / chemistry*
DNA-Binding Proteins / genetics
Gene Expression
Humans
Models, Molecular*
Molecular Sequence Data
Nuclear Proteins / chemistry*
Nuclear Proteins / genetics
Protein Binding
Protein Multimerization
Protein Structure, Tertiary
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Scattering, Small Angle
Solutions
Static Electricity
Tumor Suppressor Proteins / chemistry*
Tumor Suppressor Proteins / genetics
X-Ray Diffraction

Substances

Apoptosis Regulatory Proteins
CENPS protein, human
CENPX protein, human
Chromatin
DNA-Binding Proteins
Nuclear Proteins
Recombinant Proteins
Solutions
Tumor Suppressor Proteins
DNA
FANCM protein, human
DNA Helicases